NOTE: To jump to a particular place in the game or make your nanobot invincible, use the Instructor Menu at the start up screen. Type abc at password prompt, and then select a starting point:

• Story Intro and Basic Training
• Game 1: Monocyte to Macrophage (Transmigration)
• Enter the Connective Tissue
• Game 2: Follow the Chemical Trail (of C3a)
• Game 3: Recognize the Enemy (Activate LPS Receptors)
• Game 4: Eat the Pseudomonas!
• Introducing The Neutrophil
• Game 5: Call the Neutrophil to the Site of Infection (Activate CXCL8)
• Game 6: Transfer Abilities to Neutrophils
• Game 7: Eat the Staphyloccocus

Story Intro

Our protagonist’s immune system is non-functional.

Basic Training: Navigation

Learn to control the nanobot.

Game 1: Monocyte to Macrophage (Transmigration)

Train monocytes to transmigrate out of the blood vessel network
and pass into the connective tissue.

Gameplay:

1. Activate 6 selectins to slow down the monocyte.
2. Activate 1 ICAM to stop the monocyte and initiate transmigration.
3. Watch the monocyte pass through the blood vessel wall and enter the
   connective tissue.
4. Repeat above steps for second monocyte.

Game 2: Follow the Chemical Trail (of C3a)

Train the macrophage to follow the chemical trail of C3as leading to the site of infection (the Pseudomonas bacteria).

Gameplay:

• Mark a path of C3as leading to the bacteria.

Game 3: Recognize the Enemy (Activate LPS Receptors)

Train the macrophage to recognize the Pseudomonas bacteria.

Gameplay:

• Mark 10 LPS receptors on the macrophage.

Game 4: Eat the Pseudomonas!

Target the Pseudomonas cells and watch the macrophage eat them!

Gameplay:

• Mark all of the Pseudomonas cells.

Introducing the Neutrophil

What is a neutrophil?

Game 5: Call the Neutrophil to the Site of Infection (Activate CXCL8)

Call for neutrophil back up by releasing CXCL8 chemical signals.

Gameplay:

• Mark seven CXCL8 molecules on the macrophage. They will release a CXCL8 chemical signal.

Game 6: Transfer Abilities to Neutrophils

Train the neutrophil by transferring abilities from the macrophage.

Gameplay:

• Click on each of the small circles to transfer transmigration, follow the chemical trail, and eating skills.

Game 7: Eat the Staphyloccocus

Train the neutrophil to recognize and attack the Staphylococcus.

Gameplay:

• Mark all of the Staphylococcus cells.